WebThe Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The Sec61 complex forms a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins - alpha, beta, and gamma. Web7 Jan 2024 · GFP- SEC61B. catalog : 121159. more info or order : Addgene product webpage. citations: 3. Reference; Wu L, Liu L, Xu B, Huang D, Chen X. In vitro and in vivo assay of the ER lipid scramblase TMEM41B. STAR Protoc. 2024;3:101333 pubmed publisher .
mRNA encoding Sec61β, a tail-anchored protein, is …
WebCells were cotransfected with GFP-Rab5, BFP-Sec61b, and mCh-FAM21. In dynasore-treated cells, FAM21 localized to patches and punctate structures along tubular endosomes (Fig-ure 2B). Line-scan analysis demonstrated that FAM21-labeled structures corresponded to positions where Rab5 fluorescence wasminimal,indicativeofaconstriction ... Web22 Nov 2024 · In Sec61β-depleted COS-7 cells, splicing of XBP1 mRNA (indicative of IRE1 activation) was detected (Fig. 3 A). The level of splicing was more prominent when … order a duplicate id online nc
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Web8 Mar 2014 · GFP-Sec61b (plasmid 15108), STIM1-YFP (plasmid 19754), Orai1-Myc (plasmid 21638), Orai1-YFP (plasmid 19756), and Orai1-CFP (plasmid 19757) were obtained from Addgene. mCherry-STIM1 was kindly provided by Dr Richard S Lewis (Stanford University, Palo Alto, CA). To generate GFP-RTN4b and GFP-RHD, RTN4b and RHD were amplified by … WebLabelling of the ER membrane by transfection of Sec61b-GFP also showed a dramatic ER expansion in Mfn2 KO cells in response to Tg (Figure 1C). However, labelling of ER … WebThis probe colocalized with Sec61b-GFP (Figure S9c and d) and when visualized using STED could resolve individual ER tubules with a FWHM of ~50 nm (Figure 1g and h). The ER HIDE probe generated using Yale595-Tz was then used in combination with DiI-N 3/SiR-DBCO to achieve two-color HIDE imaging of the ER and PM. HeLa cells were treated iraq chemical attack on iran