Phis2

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August undefined, 2024

Webb27 mars 2015 · To suppress potential background activity, the medium was complemented with 50 or 100 m m 3-amino-1,2,4-triazole (3-AT) and yeast strains harbouring recombinant pHis2.1 were plated on this medium in different dilutions. In case of pHis2.1-Seq15 containing 4CRM-DJ1E, 50 m m 3-AT was sufficient to suppress background growth. Webb10 aug. 2015 · The yeast one-hybrid assay was carried out using the Matchmaker one-hybrid system following the manual (Clontech, Palo Alto, CA, USA). The promoter …

Transcription Factor-Centered Yeast One-Hybrid Assay

WebbpHIS2.1 DNA target TRP1 Kanr HIS3 Figure 2. One-hybrid library construction and screening. Your target bait sequence is cloned upstream of the His3 reporter in pHIS2.1. … WebbPhis2 1 Bait Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … great northern school district washington

Self-activation detection of pHis2-element recombinant vectors ...

Webb19 sep. 2024 · These sequences were cloned into pHIS2 (Clontech, Palo Alto, CA, USA) upstream of the reporter gene HIS3. Co-transformation of pHIS2 with or without the TCP cis-element sequences and GbTCP4-pGADT7 into Y187 cells was performed as described by Yu et al. (2024) . The pGADT7 vector carries the LEU2 nutritional marker for selection … WebbpHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication se-quence ( ARS4) and TRP1 nutritional marker for replication and selection in yeast (1, … WebbGene. Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, … great northern seafood company

Phis2 TaKaRa Bioz

WebbpHis1, pHis2, pHis3 pGST1, pGST2, pGST3 pMBP1, pMBP2, pMBP3: pET44a-c: Novagen: T7 promoter, N-term his tag followed by NusA fusion protein, another his-tag, S-tag, … Webb21 mars 2024 · 苹果腐烂病是苹果生产中最具破坏性的病害。. 在苹果树上添加钾可以有效地控制这种疾病。. 然而，苹果在高钾状态下对苹果腐烂病抗性的潜在机制仍然未知。. 在这里，我们发现高K（HK，9.30 g/kg）苹果组织表现出较高的抗病性。. 当阻断K通道时，阻力受 … floor function wolframhttp://www.honorgene.com/uploads/files/202411/6181dfe1813d2.pdf floor function wolfram alpha

"Webb1 mars 2024 · MdGSTF6-p-pHIS2 vectors were first grown on −Trp/−His medium containing 3-amino-1,2,4-triazole (3-AT) for screening. The concentration of 3-AT used … " - Phis2

Phis2

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Webb2 nov. 2024 · This Public Health Inspection System (PHIS) Industry User Guide is designed for all industry representatives who produce and sell meat, poultry, and egg products … http://www.labbase.net/Supply/SupplyItems-4229347.html

WebbpHIS2 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for … WebbUSD $185.00. Matchmaker Insert Check PCR Mix 1 is a 2X pre-mix that allows quick and easy PCR directly from yeast colonies. The Mix contains a PCR polymerase, primers, …

WebbpHIS-Hsv2 was a gift from James Hurley (Addgene plasmid # 42524 ; http://n2t.net/addgene:42524 ; RRID:Addgene_42524) For your References section: Two … Webb6 feb. 2024 · The chloroplast comprises the outer and inner membranes that are composed of the translocon protein complexes Toc and Tic (translocon at the …

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WebbDescription. District Health Information Software 2 Tracker (DHIS2 Tracker) is an open-source, web-based application that supports data collection, and analysis of … floor framing under a bathtubWebb9 mars 2024 · Use the outward primers phiS2 and psiR3 together with a DNA polymerase not inhibited by. bacterial cultures such as TAQ polymerase for the PCR and sequence the insert with the nested reverse primer psiR2. Use. less than 1 l of bacterial culture supernatant per 50 l-PCR reaction or the touch of a toothpick on a colony as DNA. great northern seed companyWebb26 maj 2024 · pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for … floor function sqlWebbStack Exchange network consists of 181 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, … great northern shipping company incWebbY187-pHis2YeastOne-HybridMediaKit（酵母单杂培养基套装YM1010），含有用于Y187-pHis2系统酵母单杂交文库筛选的全套培养基，性价比更高。培养基以非常方便使用的 … floor function of xWebb14 aug. 2015 · 产品报价：询价更新时间：2015/8/14 10:48:01 产地：广东. 品牌：伟通生物. 型号：各种质粒载体. 厂商性质：生产型,贸易型,服务型, 公司名称：深圳市伟通生物科技有限公司产品关键词： great northern sfWebb29 okt. 2024 · To test whether CmMYB9a directly binds to the promoters of these four structural genes, yeast one-hybrid assays were performed with co-transformation of pGADT7 (AD)-CmMYB9a constructs and pHIS2 vectors containing the promoters. Co-transformation of the AD vector and these pHIS2 constructs served as negative controls. floor furnace repair in memphis