Clonexpress multis one step cloning kit 说明书
WebMay 18, 2015 · The two purified fragments were mixed and ligated using ClonExpress MultiS One Step Cloning Kit (Vazyme, China) according to the manufacturer’s protocol. The resulting products were transformed into E. coli DH5α and transformants were selected on LB-Kan. PCR was employed to confirm the presence of sacB gene. The sucrose … WebClonExpress® One Step Cloning Kits were purchased from Vazyme Biotech Co., Ltd China. Genome and transcriptome sequencing Talaromyces sp. F08Z-0631 was performed at Novogene Co., Ltd. (China). ... digested pTAex3 using the ClonExpress® MultiS One Step Cloning Kit. For constructing plasmids harboring two or three genes, DNA …
Clonexpress multis one step cloning kit 说明书
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WebMay 7, 2024 · Assemble the DNA fragments recovered in steps 1 and 2 together by ClonExpress™II one step cloning kit, which produces two plasmids pDTg1-GeneA and pDFg1-GeneB separately. The transformation of competent E. coli TOP10 cells and the selection of positive clones using ampicillin follow the protocol described in Subheading … WebAmplification will generate blunt-ended products, which are compatible with ClonExpress II One Step Cloning Kit Series (i.e. Vazyme, #C112, #C113, #C115). Product Manual (pdf) Advantages. ... ClonExpress II One Step Cloning Kit (Vazyme, #C112) ClonExpress MultiS One Step Cloning Kit (Vazyme, #C113) ChamQ Universal SYBR qPCR Master …
WebProduct Introduction. Mut Express® MultiS Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit, three to five discrete sites on the target plasmid can be introduced at one time. The kit consists of two The ultra-high fidelity of Phanta® Max significantly reduces the ... WebCellagen Tech
WebJun 17, 2024 · Application Note: ClonExpress Ultra One Step Cloning Kit17 June 2024. Application Note: ClonExpress Ultra One Step Cloning Kit. In this application note, … WebThe cloning products can then be directly transformed to competent cells with a positive rate > 95%. ClonExpress MultiS One Step Cloning Kit is a new version cloning kit …
Webyou should design two expression cassette（contains two different promoters. for the cloning ，I recommend ClonExpress MultiS One Step Cloning Kit（vazyme）. Cite 11th Oct, 2024
WebProduct Description. The Mut Express II Fast Mutagenesis Kit V2 is designed for rapid site-directed mutagenesis based on ClonExpress rapid cloning technology. The PCR amplified target fragments are treated with DpnI to remove the template DNA, and then cyclized and transformed into E. coli to complete the site-directed mutagenesis. provincetown dentistWebJun 9, 2024 · 利用ClonExpress®II One Step Cloning Kit 非连接酶依赖型单片段快速克隆试剂盒. ClonExpress®技术是一种高效的 DNA 无缝克隆技术，可将插入片段定向克隆至任意 … restaurants in hutchinson mnWebClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Use any method to linearize … provincetown dining